Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1975 5
1976 1
1977 1
1978 1
1980 5
1981 2
1982 2
1984 1
1985 3
1986 2
1987 1
1988 1
1989 6
1990 2
1992 4
1993 5
1994 2
1995 5
1996 1
1997 4
1998 3
1999 2
2000 2
2001 3
2002 3
2003 4
2004 3
2005 1
2006 6
2008 1
2009 2
2010 2
2011 1
2012 2
2013 2
2014 3
2015 1
2016 6
2017 3
2018 3
2019 2
2020 3
2023 1
2024 0

Text availability

Article attribute

Article type

Publication date

Search Results

109 results

Results by year

Filters applied: . Clear all
Page 1
Investigation of guanidinium chloride-induced unfolding pathway of sphingosine kinase 1.
Gupta P, Khan FI, Ambreen D, Lai D, Alajmi MF, Hussain A, Islam A, Ahmad F, Hassan MI. Gupta P, et al. Int J Biol Macromol. 2020 Mar 15;147:177-186. doi: 10.1016/j.ijbiomac.2020.01.023. Epub 2020 Jan 7. Int J Biol Macromol. 2020. PMID: 31917989
In the present study, we investigated the guanidinium chloride (GdmCl)-induced denaturation of SphK1 at pH 8.0 and 25 C using two different spectroscopic probes, i.e., mean residue ellipticity at 222 nm ([teta](222)) and fluorescence emission maxima (lambda(m …
In the present study, we investigated the guanidinium chloride (GdmCl)-induced denaturation of SphK1 at pH 8.0 and 25 C using two different …
α-Conotoxin Decontamination Protocol Evaluation: What Works and What Doesn't.
Turner MW, Cort JR, McDougal OM. Turner MW, et al. Toxins (Basel). 2017 Sep 14;9(9):281. doi: 10.3390/toxins9090281. Toxins (Basel). 2017. PMID: 28906461 Free PMC article.
Of the nine decontamination methods tested, treatment with 1% (m/v) solution of the enzymatic detergent Contrex EZ resulted in a 76.8% decrease in alpha-helical content as assessed by the mean residue ellipticity at 222 nm, and partial peptide digestion was d …
Of the nine decontamination methods tested, treatment with 1% (m/v) solution of the enzymatic detergent Contrex EZ resulted in a 76.8% decre …
Renaturation of Myoglobin Denatured by Sodium Dodecyl Sulfate by Removal of Dodecyl Sulfate Ions Bound to the Protein Using Sodium Cholate.
Moriyama Y, Takeda K. Moriyama Y, et al. J Oleo Sci. 2023 Mar 30;72(4):481-487. doi: 10.5650/jos.ess22396. Epub 2023 Mar 10. J Oleo Sci. 2023. PMID: 36908176 Free article.
The extent of removal of DS ions was estimated by the restoration of the secondary and tertiary structures of the protein disrupted by SDS. The secondary structural change was followed by monitoring mean residue ellipticity at 222 nm, [teta](222), which was f …
The extent of removal of DS ions was estimated by the restoration of the secondary and tertiary structures of the protein disrupted by SDS. …
Changes in biophysical characteristics of PFN1 due to mutation causing amyotrophic lateral sclerosis.
Nekouei M, Ghezellou P, Aliahmadi A, Arjmand S, Kiaei M, Ghassempour A. Nekouei M, et al. Metab Brain Dis. 2018 Dec;33(6):1975-1984. doi: 10.1007/s11011-018-0305-4. Epub 2018 Sep 10. Metab Brain Dis. 2018. PMID: 30203378 Free PMC article.
Initially, we expressed and studied the purified PFN1(WT) and PFN1(G118V) proteins from bacterial culture. We found that the PFN1(G118V) protein has a different mean residue ellipticity, as measured by far-UV circular dichroism, accompanied by a spectral shif …
Initially, we expressed and studied the purified PFN1(WT) and PFN1(G118V) proteins from bacterial culture. We found that the PFN1(G118V) pro …
Removal of Dodecyl Sulfate Ions Bound to Human and Bovine Serum Albumins Using Sodium Cholate.
Moriyama Y, Takeda K. Moriyama Y, et al. J Oleo Sci. 2020;69(1):65-72. doi: 10.5650/jos.ess19224. J Oleo Sci. 2020. PMID: 31902896 Free article.
Increases and decreases in alpha-helical structure were examined using the mean residue ellipticity at 222 nm, [teta](222), which was frequently used as a measure of alpha-helical structure content. ...
Increases and decreases in alpha-helical structure were examined using the mean residue ellipticity at 222 nm, [teta](2 …
Electrostatic interactions at the interface of two enzymes are essential for two-step alkane biosynthesis in cyanobacteria.
Chang M, Shimba K, Hayashi Y, Arai M. Chang M, et al. Biosci Biotechnol Biochem. 2020 Feb;84(2):228-237. doi: 10.1080/09168451.2019.1677142. Epub 2019 Oct 10. Biosci Biotechnol Biochem. 2020. PMID: 31601165
Here, using analytical size-exclusion chromatography (SEC), we show that electrostatic interactions play an important role in the binding of the two enzymes. Alanine-scanning mutagenesis at charged residues around the substrate entry site of ADO revealed that E201A mutatio …
Here, using analytical size-exclusion chromatography (SEC), we show that electrostatic interactions play an important role in the binding of …
Characterisation of molten globule-like state of sheep serum albumin at physiological pH.
Dar MA, Wahiduzzaman, Haque MA, Islam A, Hassan MI, Ahmad F. Dar MA, et al. Int J Biol Macromol. 2016 Aug;89:605-13. doi: 10.1016/j.ijbiomac.2016.05.036. Epub 2016 May 11. Int J Biol Macromol. 2016. PMID: 27180298
Sheep serum albumin (SSA) is a 583 amino acid residues long multidomain monomeric protein which is rich in cysteine and low in tryptophan content. ...Further, we have studied urea-induced denaturation of SSA by monitoring changes in the difference absorption coefficient at …
Sheep serum albumin (SSA) is a 583 amino acid residues long multidomain monomeric protein which is rich in cysteine and low in trypto …
Purification, preliminary X-ray crystallography and biophysical studies of triose phosphate isomerase-β-globin subunit complex.
Wahiduzzaman, Dar MA, Amir M, Islam A, Hassan MI, Ahmad F. Wahiduzzaman, et al. Int J Biol Macromol. 2017 Jan;94(Pt B):746-753. doi: 10.1016/j.ijbiomac.2016.10.070. Epub 2016 Oct 22. Int J Biol Macromol. 2017. PMID: 27780759
We further studied guanidinium chloride (GdmCl)-induced denaturation of TIM-beta-globin complex by monitoring changes in the mean residue ellipticity at 222nm ([teta](222)) and difference absorption coefficient at 406nm (deltaepsilon(406)) at pH 7.5 and 25C. …
We further studied guanidinium chloride (GdmCl)-induced denaturation of TIM-beta-globin complex by monitoring changes in the mean …
Thermodynamics of the self-association of glucagon.
Formisano S, Johnson ML, Edelhoch H. Formisano S, et al. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3340-4. doi: 10.1073/pnas.74.8.3340. Proc Natl Acad Sci U S A. 1977. PMID: 269394 Free PMC article.
In water, glucagon exists in an equilibrium between a trimer in which more than half of the peptide groups are in an alpha-helical configuration and a monomer which has a random coil configuration with few alpha-helical residues. The thermodynamics of this self-association …
In water, glucagon exists in an equilibrium between a trimer in which more than half of the peptide groups are in an alpha-helical configura …
Residue helix parameters obtained from dichroic analysis of peptides of defined sequence.
Park SH, Shalongo W, Stellwagen E. Park SH, et al. Biochemistry. 1993 Jul 13;32(27):7048-53. doi: 10.1021/bi00078a033. Biochemistry. 1993. PMID: 8334134
The changes observed in the dichroic spectra are characteristic for a two-state helix/coil transition. The mean residue ellipticity at 222 nm exhibits a curvilinear dependence on ionic strength which becomes linear at ionic strengths greater than 1 M. ...An e …
The changes observed in the dichroic spectra are characteristic for a two-state helix/coil transition. The mean residue ell …
109 results